Part:BBa_K1298003:Design
PcChia 1-1 (insert) using J04500
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 745
Illegal AgeI site found at 671 - 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 229
Design Notes
With the PcChia 1-1 protein code, illegal cut sites were removed using DNA optimization. There is also no secretory tag; the chitinase protein being produced is not being secreted outside of the cell, but staying inside.
Source
BBa_K1298000 (PcChia 1-1)
- Phytochemistry paper of Dr. Kolosova (1)
- NCBI (protein code) (2)
- Bio Basic Inc. (DNA optimization)
References
1. Kolosova, N. “Cloning and characterization of chitinases from interior spruce and lodgepole pine.” 1 Oct. 2013. Feb. 2014 <http://www.ncbi.nlm.nih.gov/pubmed/24564978>
2. Kolosova, N. "Pinus contorta class I chitinase (Chia1-1) mRNA, complete cds." 30 Dec. 2011. Feb. 2014 <http://www.ncbi.nlm.nih.gov/nuccore/HM219849>*
*The sequence found at this link has been optimized to remove illegal cut sites (EcoRI, XbaI, SpeI, PstI) and therefore is not the same as the sequence found on this part page.